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Fig. 1
Transgenic Gene Expression in the Sympathetic Neurons and the Interrenal Gland (A) Left: Schematic of a transverse section illustrating zebrafish anatomical structures, dorsal upward. Right: Schematic of a sagittal section illustrating zebrafish anatomical structures, anterior to left. (B) EGFP expression (green) in the zebrafish chain of sympathetic ganglia (arrowheads), the IRG (arrow), and medulla oblongata (asterisk) at 3 wpf. Lateral view of confocal-brightfield image, anterior to left. The magnified view of the boxed region is shown on the right. Scale bar represents 500 μm. (C) EGFP is coexpressed with TH in the SCG at 6 wpf (sagittal section); TH coexpression is indicated in red. Scale bar represents 20 μm. (D) EGFP is coexpressed with TH in the chain of sympathetic ganglia at 6 wpf (transverse section). TH coexpression is indicated in red. Scale bar represents 20 μm. (E) EGFP is coexpressed with TH in the IRG at 8 months postfertilization (mpf) (sagittal section). TH coexpression is indicated in red. Scale bar represents 100 μm. Ao, aorta; Cb, cerebellum; Chfn, chromaffin cells; E, esophagus; G, gill; H, heart; HK, head kidney; I, intestine; IRG, interregnal gland; KB, kidney body; L, liver; Me, medulla; Nc, notochord; SB, swim bladder; SC, spinal cord; SCG, superior cervical ganglion; Sym.C, sympathetic chain. See also Figure S1.
Reprinted from Cancer Cell, 21(3), Zhu, S., Lee, J.S., Guo, F., Shin, J., Perez-Atayde, A.R., Kutok, J.L., Rodig, S.J., Neuberg, D.S., Helman, D., Feng, H., Stewart, R.A., Wang, W., George, R.E., Kanki, J.P., and Look, A.T., Activated ALK Collaborates with MYCN in Neuroblastoma Pathogenesis, 362-373, Copyright (2012) with permission from Elsevier. Full text @ Cancer Cell