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Fig. S9 Expression of marker genes for cardiac chamber and atrioventricular canal development are unchanged in popdc2 morphants. Whole mount in situ hybridization analysis of (A,B) cmlc2, (C,D) vmhc, (E,F) bmp4, and (G,H) notch1b expression in (A,C,E,G) control (CTR) and (B,D,F,H) MO1-popdc2 (MO1) morphants at (A–D) 72 hpf and (E–H) 54 hpf, respectively. (A–D) No obvious differences in chamber dimensions were observed between control and MO1-popdc2 morphants. Note the lack of vmhc-stained facial muscles (fm) in the MO1-popdc2 morphant (arrowheads in D). (E–H) Expression of the myocardial atrioventricular canal (AVC) marker gene bmp4 is observed in the inflow tract, AVC and outflow tract and no difference was observed between control embryos and popdc2 morphants. (G,H) Likewise, the endocardial marker notch1b is expressed in the outflow tract and in the AVC with similar intensity in MO-control and MO1-popdc2 injected embryos, respectively. Abbreviations: a—atrium; v—ventricle.
Reprinted from Developmental Biology, 363(2), Kirchmaier, B.C., Poon, K.L., Schwerte, T., Huisken, J., Winkler, C., Jungblut, B., Stainier, D.Y., and Brand, T., The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development, 438-450, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.