|
Fig. S6 Aberrant craniofacial skeletal muscle development in popdc2 morphants. Confocal microscopy of whole mount immunostained embryos using MF20 antibody. Embryos were injected with (A, C, E, G) control (MO-CTR), or (B, D, F, H) MO1-popdc2 morpholinos, respectively. Specimens are viewed from (A–D) the left lateral side, or are (E–H) ventral views. Many of the craniofacial muscles were malformed and reduced in size in the popdc2 morphants. Abbreviations: ah, adductor hyoideus; am, adductor mandibulae; ao, adductor operculi; do, dilator operculi; dpw1–5, dorsal pharyngeal wall 1–5; hh, hyohyoideus; ih, interhyoideus; ima, intermandibularis anterior; imp, intermandibularis posterior; io, inferior oblique; ir, interior rectus; lap, levator arcus palatini; lr, lateral rectus; mr, medial rectus; sh, sternohyoideus; so, superior oblique; sr, superior rectus; tv 1–5, transversus ventralis 1–5; v, ventricle.
Reprinted from Developmental Biology, 363(2), Kirchmaier, B.C., Poon, K.L., Schwerte, T., Huisken, J., Winkler, C., Jungblut, B., Stainier, D.Y., and Brand, T., The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development, 438-450, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.