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Fig. 5
The Cx40.8-b domain is responsible for the intracellular localization of Cx40.8.
HeLa cells were singly transfected with EGFP fusions of each construct. (A) Cx43-BCD-EGFP, (B) Cx40.8-bcd-EGFP, (C) Cx43-bcd-EGFP, (D) Cx40.8-BCD-EGFP, (E) Cx43-bCD-EGFP, (F) Cx40.8-Bcd-EGFP, (G) Cx43-BcD-EGFP, (H) Cx40.8-bCd-EGFP, (I) Cx43-BCd-EGFP, (J) Cx40.8-bcD-EGFP, (K) Cx43-bBCD-EGFP, (L) Cx40.8-cd-EGFP. Asterisks denote constructs in which the localization depends on a component of the carboxy terminus. Arrows identify gap junction plaques.
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