|
Fig. S4
Effects of the 52cap, poly(A) tail and Paip2 on translation in zebrafish embryos. (A) The effect of the 52 cap and poly(A) tails on Rluc mRNA translation in zebrafish embryos. Rluc activity was measured at 6 hpf and normalized to the Fluc activity. The normalized Rluc activity with the m7GpppG cap and poly(A) was set to one. The data show the averages of three independent experiments. Error bars show SD. (B) The effect of Paip2 on poly(A) tail-stimulated translation. Rluc activity of poly(A)- Rluc mRNA was set to one. Western blot detecting Myc-tagged GFP or Paip2 is shown at the bottom. (C) The qRT-PCR analysis of reporter mRNA stability of Fig. 3F. The normalized Rluc mRNA values [normalized to those of the HA-λN empty construct (HA-N)] were set to one. The data show averages of three independent experiments. Error bars show SD.