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Fig. S3 The splice-blocker morpholino targeting eif3hb pre-mRNA retains specificity at high dosage. Reverse transcriptase-polymerse chain reaction (RT-PCR) assays were carried out using total RNA isolated from eif3hb morphant embryos targeted with 4 or 5 ng of splice-blocker morpholino to measure the relative levels of both eif3ha and eif3hb mature mRNAs. Under these conditions, eif3hb morphants showed severe brain degeneration phenotypes. A wild-type control (WT) was also included. β-actin (actin) was used as an internal control.
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