Fig. S1

Fig. S1

Induction of Dkk-GFP and Wnt8-GFP expression from transgenic lines at the 16 s stage. As previously reported (Stoick-Cooper et al., 2007; Weidinger et al., 2005), both the Dkk and Wnt8 used for the transgenic lines are tagged with GFP facilitating the easy detection of transgenic embryos. (A,C) HCSEs. (B) Representative GFP + Tg(hsp70l:dkk1-GFP)^w3 embryo 30 min after the heat-shock. For the Tg(hsp70l:dkk1-GFP)^w3 embryos, fluorescence was maximal at ~ 1 h. (D) Representative GFP + Tg(hsp70l:wnt8a-GFP)^w34 embryo 2 h after heat-shock. For the Tg(hsp70l:wnt8a-GFP)^w34 embryos, fluorescence was maximal at ~ 2 h post-heat shock. For both transgenes, GFP expression could be detected by 30 min post-heat-shock. Overall, relative to each other, the fluorescence induced from the Dkk-GFP transgene is consistently brighter than the Wnt-GFP transgene (data not shown). Equivalent results were obtained with heat-shocks performed at the TB stage. Fluorescence in the yolk in all images is autofluorescence.