ZFIN Image: Neto et al., 2012, Fig. 9

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Figure Caption

Fig. 9

Temporal requirement of RA for tbx5a, wnt2b and Osr expression. Dorsal views of embryos at the 21- to 24-somite stage treated with DEAB from the time shown above the panels showing the expression of genes indicated on the left. (A,E,I,M,Q) Control embryos incubated with DMSO. (B,F,J,N,R) Interference with RA signaling from 50% epiboly caused strong downregulation of all genes examined. (C,G,K,O,S) Incubation with DEAB from tailbud caused strong downregulation of tbx5a, osr1, wnt2ba and wnt2bb (C,G,O,S, respectively) while not affecting osr2 expression (K). (D,H,L,P,T) Culturing embryos with DEAB from the 6-somite stage only caused some tbx5a downregulation (D). Arrowheads indicate the tbx5a-expressing domain (A-D) and the kidney anlage (E-T).

Figure Data

Expression / Labeling details

Acknowledgments

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