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Fig. 6 BAC dissection to detect otx1b non-conserved enhancer. (A) The Otx1b 111 kb BAC showing the gene and the 6.4 kb non-conserved functional element and the CNEs present. The blue lines indicate the XbaI restriction sites. The plus sign in parenthesis indicates functional CNEs. (B) Six overlapping PCR fragments covering the 6.4 kb fragment. (C) Five overlapping PCR fragments covering the 1050 NC2 functional fragment (D) the seven Cy5 labeled probes designed on the functional NC2C fragment. Transgenic assay with the whole BAC (E), 6.4 kb fragment (F), NC2 (1050 bp) fragment (G) and NC2C (210 bp) fragment (H) all at 24 hpf.