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Fig. 4 Depletion of zebrafish Nod1 or Nod2 results in reduced expression of duox and increased S. enterica burden following infection in larvae. (A) Expression of duox examined by qPCR at 6 and 24 hpi. Data represents at least triplicate pools of 10 to 20 larvae. Error bars represent s.e.m.; statistically significant differences of P<0.05 (*), P<0.01 (**) and P<0.0001 (***) as determined by ANOVA are marked. (B) Expression of duox in 4-dpf control and morphant larvae examined by in situ hybridization. Green arrows indicate thyroid expression; red arrows indicate intestinal epithelial cell expression. (C) Whole-larvae and intracellular bacterial recovery from 6 and 24 hpi larvae. Triplicate groups of five larvae were analyzed for each biological replicate represented by a single dot. Error bars represent s.e.m.; statistically significant differences of P<0.05 (*) and P<0.01 (**) as determined by ANOVA are marked.