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Fig. S6 Gli-targeting MOs modulate Hh pathway state and PMN formation without perturbing RB neuron development. (A-O) MZsmo embryos were uninjected (A-C) or injected with MOs targeting gli2b (D-F), gli1 (G-I), gli2a/gli2b/gli3 (J-L) or gli2a/gli3 (M-O) and then grown to the 18-somite stage (18 hpf), fixed, and stained for (A,B,D,E,G,H,J,K,M,N) ptc1 transcripts to assess Hh pathway state or (C,F,I,L,O) isl2a transcripts to visualize ventrally positioned PMNs and dorsally positioned RB sensory neurons. Embryos are viewed laterally, anterior to the left, and the trunk region spanning approximately the eighth and tenth somites is shown. Knockdown of gli2b diminishes neural tube ptc1 expression in the trunk but expands it slightly in the hindbrain. Scale bar: 50 μm.