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Fig. 2 Expression of Nsf in the CNS and lateral line organ (5 dpf).
A, An antibody against human NSF protein labels the larval nervous system (5 dpf). B, Labeling is absent in nsfst53 mutants. Images are projections of 5 optical sections of 10 μm thickness. Scale bar, 100 μm. C-F, Confocal projections (10×1 μm sections) of anti-NSF (red) and Ribeye b (Blue) in TgBAC(neurod:EGFP)nl1 transgenic fish. Nsf signal colocalized with anti-GFP (green) in the afferent nerve, and was also in Ribeye b-positive hair cells. Scale bar, 10 μm.
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