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Fig. 4 Retinoic acid signaling promotes motoneurogenesis and basal Gli activity. (A) Quantification of isl2a-positive PMNs at 18 hpf after treatment with DEAB and/or RA. Error bars represent s.e.m. ***, P<0.001. (B-G) Wild-type and MZsmo zebrafish embryos (14 hpf) were treated with DMSO, 10 μM DEAB or 100 nM RA and stained for ptc1 transcripts to assess the Hh pathway state. Embryos are flat-mounted, anterior to the left and viewed dorsally, with arrowheads demarcating the neural tube region. (H,I) Quantification of gli2b expression in the neural tubes of wild-type or MZsmo embryos treated with DMSO, 10 μM DEAB or 100 nM RA and then stained for gli2b transcripts. Data are the average of at least five embryos per condition ± s.e.m. (J-O) Representative images of embryos treated, stained and quantified as described for H,I, with the analyzed regions highlighted in white brackets. Scale bars: 200 μm.