ZFIN Image: Mich et al., 2011, Fig. S8

Image

Figure Caption

Fig. S8 RA signaling promotes PMN induction in the absence of Hh signaling. (A-F) Wild-type or MZsmo embryos were treated with DMSO, DEAB (10 μM) or RA (100 nM) from the one-cell stage to the 18-somite stage and then fixed. The embryos were then stained for isl2a transcripts to mark caudal and variable PMNs (primary micrographs) or both isl1 and isl2a transcripts to mark all PMNs (insets). Inset images are high magnification micrographs of the region spanning approximately the ninth and eleventh somites, where isl1 does not label non-PMN neurons in wild-type embryos. Dashed boxes denote PMNs within each inset image. All embryos are viewed laterally, anterior to the left. Scale bars: 200 μm in primary micrographs; 20 μm in insets.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development