Fig. S3

Fig. S3 BMPs fail to induce dlx3b in the absence of both Edn1 and Hand2. (A-C) Confocal sections of in situ hybridizations at 36 hpf for dlx3b (red) and hand2 (green). In controls injected with hand2-MO, a slight ventral expansion of dlx3b was seen (A, n=12) compared to un-injected embryos (see Fig. 5A). UAS:Bmp4 embryos subjected to a three-minute heat-shock treatment and injected with hand2-MO show expansion of dlx3b and hand2 (B, n=7), yet no upregulation of dlx3b was observed in UAS:Bmp4; edn1^/ embryos injected with hand2-MO (C, n=5). Scale bar: 50 μm.