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Fig. 4
Photoperiod length influences melanopsin expression.
(A, C) Larvae housed in 14:10 LD or (B,D) 18:6 LD cycles were fixed at ZT1 at 96 hpf, and assayed for opn4-related gene expression. opn4a and opn4.1 expression in the inner nuclear layer is greatly reduced in the prolonged light conditions. Additionally, opn4.1 transcripts are only detected in the photoreceptor cells (arrowhead) of larvae raised in the 14:10 cycle. Over 90 larvae were assayed in 3 independent experiments.
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