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Fig. 3 Hdac1 acts epistatically and in opposition to the inhibitory effect of Notch signalling on erm transcription in the hindbrain ventricular zone. In situ hybridisation analysis of erm expression in (A,E,I) wild-type sibling embyos injected with the hdac1 mismatch control morpholino, (B,F,J) wild-type sibling embryos injected with the hdac1ATG1 morpholino, (C,G,K) mind bomb mutant embryos injected with the hdac1 mismatch control morpholino, and (D,H,L) mind bomb mutant embryos injected with the hdac1ATG1 morpholino. Panels show lateral views (A-D), dorsal views of hindbrain (E-H), and transverse sections through rhombomere 5 (I-L) of 27 hpf embryos. Expression of erm is almost completely extinguished in the hindbrain of both hdac1ATG1 morphant embryos (B,F,J) and mind bomb mutants microinjected with the hdac1ATG1 morpholino (D,H,L), whereas the erm expression domain is expanded in the hindbrain of mind bomb mutants (C,G,K), and remains restricted to stripes at rhombomere centres in wild-type siblings microinjected with the hdac1 mismatch control morpholino (A,E,I).