Fig. 7

Fig. 7 The function of Six1a as a transcriptional repressor in the neuronal lineage of the developing zebrafish inner ear likely involves Hdac1 and Gro1. Loss of function of either hdac1 or gro1 leads to a decrease in number of neurons similar to that observed when six1a is over-expressed. Effects of perturbing these molecules are restricted to the neuronal lineage. Average number of hair cells and neurons in 3 dpf utricular maculae and SAG. Hair cells and neurons were detected by HCS-1 and HuC immunolabeling, respectively, using confocal microscopy. Values represent mean cell counts (± standard deviation) with a sample size of 20 for each experiment. Statistical analyses were performed for both panels with Student′s t test; all comparisons were made to embryos injected with standard MO control (STD) or with six1a mRNA. Comparisons where P < 0.05 were considered statistically significant.