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Fig. S7 kcnq1 knockdown enhances loss of fluorescent dextran. Control and kcnq1 MO injected embryos were loaded with rhodamine-labeled dextran at 48 hpf. >80 embryos were analyzed for each group in 3 separate experiments (a,d) Fluorescence microscopy at 6 hpi reveals equal fluorescence loading between embryos. (b,e) At 72 hpf (24 hpi), fluorescence intensity in kcnq1 morphants is diminished. (c,f) At 96 hpf (48 hpi) control embryos retain their fluorescence, but kcnq1 morphants have significantly diminished fluorescence.
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