ZFIN Image: Pyati et al., 2011, Fig. S5

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Figure Caption

Fig. S5

related to Figure 5: Knockdown of p63 rescues the ER-stress induced apoptosis in skin epidermis of zebrafish embryos treated with brefeldin A or thapsigargin

Embryos injected with the p63 morpholino were treated either with Brefeldin A (A) or with thapsigargin (B) and the apoptosis observed in the tail epidermis was analyzed by AO staining. Cropped photos of zebrafish tail regions were quantitated for total fluorescence intensity with Volocity software, and values were normalized to controls. As shown in the graph, p63 knockdown (p63 mo) in embryos treated with Brefeldin A, rescues the ER-stress induced cell death as compared to control embryos treated with ethanol (ctrl etoh) or embryos injected with control morpholinos (ctrl mo) (A). p63 knockdown (p63 mo) in embryos treated thapsigargin rescues the ER-stress induced cell death as compared to control embryos treated with DMSO (ctrl dmso) or embryos injected with control morpholinos (ctrl mo) (B).

Acknowledgments

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Reprinted from Developmental Cell, 21(3), Pyati, U.J., Gjini, E., Carbonneau, S., Lee, J.S., Guo, F., Jette, C.A., Kelsell, D.P., and Look, A.T., p63 Mediates an Apoptotic Response to Pharmacological and Disease-Related ER Stress in the Developing Epidermis, 492-505, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell