ZFIN Image: Pyati et al., 2011, Fig. S4

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Figure Caption

Fig. S4

related to Figure 5: Knockdown of puma rescues the ER-stress induced apoptosis in skin epidermis of zebrafish embryos treated with thapsigargin.

Morpholinos against puma (A) and p63 (C) yield effective knockdown based on RT-PCR levels. (A) A decrease in the puma transcript is observed compared to the puma transcript in control morpholino injected embryos. (B) Knockdown of puma rescues the ER-stress induced apoptosis in skin epidermis of zebrafish embryos treated with thapsigargin. (C) RT-PCR levels for p63 where also decreased in embryos injected with the p63 morpholino compared to control morpholino injected embryos. (D) Knockdown of p73 causes jaw cartilage defects in zebrafish embryos, as previously reported, verifying the effectiveness of the p73 knockdown in embryos injected with the p73 morpholino and analyzed for rapid ER-stress induced apoptosis post-thapsigargin treatment (Alcian blue staining).

Acknowledgments

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Reprinted from Developmental Cell, 21(3), Pyati, U.J., Gjini, E., Carbonneau, S., Lee, J.S., Guo, F., Jette, C.A., Kelsell, D.P., and Look, A.T., p63 Mediates an Apoptotic Response to Pharmacological and Disease-Related ER Stress in the Developing Epidermis, 492-505, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell