ZFIN Image: Chang et al., 2011, Fig. 4

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Figure Caption

Fig. 4

Knockdown of Leg1a or Leg1b protein expression with their specific morpholinos.

(A) Diagram showing construction of leg1a-52-UTR:rfp (52UTRa:RFP) and leg1b-52-UTR:gfp (52UTRb:GFP) plasmids. (B–I) 52UTRa:RFP and 52UTRb:GFP were mixed and co-injected with st-MO (B, F), leg1-MO^ATG (C, G) or leg1a-MO (D, H), or leg1b-MO (E, I). Rfp (B–E) and Gfp (F–I) fluorescence was visualized under a Nikon fluorescence microscope. (J) Western blotting analysis of Rfp and Gfp proteins in the injected embryos as indicated using antibodies against Rfp and Gfp, respectively. (K) Western blotting analysis of total Leg1 in different morphants as indicated.

Acknowledgments

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