Fig. 2
Global overexpression of gata5/smarcd3b/tbx5 in zebrafish results in expanded expression of myocardial-specific genes. (A-C) Expression of nkx2.5 visualized by RNA in situ hybridization on 8-somite stage (13 hpf) wild-type (C), gata5-overexpressing (B) and cBAF-overexpressing (A) embryos. Dorsal views with anterior towards the top of images. (D-F) RNA in situ hybridization for myl7 expression at 24 hpf in wild-type (F), gata5-overexpressing (E) and cBAF-overexpressing (D) embryos. Dorsal views of the heart region are shown. (G-J) Light and fluorescent images of 24 hpf myl7:EGFP transgenic wild-type (I,J) and cBAF overexpressing (G,H) embryos. Lateral views with anterior towards the left are shown.