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Fig. 2
Expression pattern of zCyr61c-23 detected by in situ hybridization. a-n: Photographs of zebrafish embryos staged at 80% epiboly (a, dorsal view; b, lateral view), 5-8 somites (c,d,d′), and at 24 (e,e′,f), 48 (g,h), 96 (i-l), and 120 (m,n) hours postfertilization (hpf). Expression is observed in the lateral edges of the neural plate (a,b), the dorsal brain (c,d,d′,e, arrowheads), along the midline (e′,f), somites (f), pharyngeal cartilages (k, arrowhead), thyroid region (g,i-n, arrows) and in the developing hypurals (h, arrow). Double labeling of zCyr61c-23 (arrowhead) with pax2a (arrows) at five to eight somites and 24 hpf are shown in panel d´ and e, respectively. At the five to eight somite stage zCyr61-c23 is found between the midbrain-hindbrain boundary (mhb) and the otic placode (op, d′). By 24 hpf, zCyr61-c23 colocalizes with pax2a at the mhb (e, arrowhead) but is also seen extending from the anterior midbrain (mb) to the rhombomeres (rh, e, f) as evidenced by double labeling with epha4a (f, arrows). Double labeling of zCyr61-c23 with thyroglobulin shows colocalization in the thyroid region (j, l, arrows). Scale bars = 500 μm.