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Fig. 1 zCREST1:membRFP transgenic line allows enhanced visualization of cell shape and protrusions in FBMNs. A: zCREST1:membRFP transgene. Expression of membrane-targeted RFP was driven by branchiomotor neuron-specific zCREST1 enhancer of the islet1 promoter. B-D: Dorsal views of live zCREST1:membRFP; islet1:GFP double-transgenic 24 hpf embryos. B: zCREST1:membRFP transgene expression is limited to branchiomotor neurons, as well as a subset of cells in the otic vesicle (yellow arrowheads). C: islet1:GFP transgene. D: Merged images. D: Higher magnification view of region enclosed by yellow box in D, highlighting protrusions visible with membRFP expression (white arrowheads).