ZFIN Image: Paridaen et al., 2011, Fig. S7b

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Figure Caption

Fig. S7b

Statistics: Two-tailed Student′s t-test, p-values indicated above the bars (*p < 0.01, **p < 0.02). Error bars represent standard deviation. (F–I) Single-plane confocal images of clones of transplanted cells showing DAPI-counterstained nuclei (cyan, top left), streptavidin-Alexa488 (green, top right), EdU (white, bottom left) and zn8-stained RGCs (magenta, bottom right). Clones of transplanted cells are outlined by dotted lines (white or green). The retina is outlined with dashed white lines. Shown are large clones transplanted from WT donor to WT host (F) and WT donor to gnl2 mutant host (G). In addition, images of clones from WT donor to 5 ng gnl2-injected hosts (H) and 5 ng gnl2-injected donors to 5 ng gnl2-injected hosts (I) are shown. Scalebar 25 μm.

Acknowledgments

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Reprinted from Developmental Biology, 355(2), Paridaen, J.T., Janson, E., Utami, K.H., Pereboom, T.C., Essers, P.B., van Rooijen, C., Zivkovic, D., and Macinnes, A.W., The nucleolar GTP-binding proteins Gnl2 and nucleostemin are required for retinal neurogenesis in developing zebrafish, 286-301, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.