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Fig. S3
Neural plate patterning is unaffected upon blocking Her8a function. Whole-mount in situ hybridization for barhl2 (A,B), her5 (C,D) and her9 (E,F) in embryos injected with her8a capped mRNA (right column) compared to control embryos (left column). Dorsal views of whole-mount embryos are shown, anterior to the top. All three markers highlight defined neural plate territories (barhl2: transverse diencephalic domain; her5: prospective midbrain-hindbrain boundary; her9: prospective eye field, midbrain-hindbrain boundary and lateral rhombencephalic stripes, see Figure 5) and appear identically expressed in the wild-type and morphant neural plate. Abbreviations: d: diencephalon; e: eye field; mhb: midbrain-hindbrain boundary; sc: presumptive spinal cord.