ZFIN Image: Varga et al., 2011, Fig. 2

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Fig. 2

Inhibition of BMP signaling in ich embryos does not result in organizer induction. In situ hybridization with boz (A,B,C), gsc (D,E,F) and chd (G,H,I) probes in uninjected ich and bmp2bMO-injected ich embryos demonstrate that while impaired BMP signaling results in the transcriptional derepression of chd at the margins of the embryo (H,I), other characteristic organizer genes such as boz and gsc are not induced (C and F). (In A-C dome staged embryos are shown from an animal pole view, D-F and G-I present lateral views of 50% epiboly and 70% epiboly staged embryos, respectively. For wild-type embryos, dorsal is on the left.)

Acknowledgments

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