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Fig. 5
Reduction in glucagon-expressing enteroendocrine cell number is detected in klf4a morphants.
glucagon RNA probe is used in whole-mount in situ hybridization to detect enteroendocrine cells in 96-hpf (A) wild type, (B) klf4a-5mmMO1- (C) klf4a-5mmMO2-injected, and (D) klf4a-MO1 and klf4a-MO2-injected embryos. (E) Quantification of glucagon-expressing enteroendocrine cell number in 96-hpf embryos with different treatments. Error bars indicate the standard error. Student′s t-test was conducted to compare klf4a morphants with either wild type, klf4a-5mmMO1-, or klf4a-5mmMO2-injected embryos.
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