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Fig. S1
Defects caused by overexpression of FoxH1, sur, and mid mRNA. Representative images of common phenotypes observed upon FoxH1, sur, and mid overexpression in wild-type embryos. (A) Embryo injected with 100 pg mid mRNA exhibiting a wild-type appearance at 24 hpf. (B) Embryo injected with 100 pg mid mRNA exhibiting a wavy notochord. (C) Embryo injected with 50 pg FoxH1 mRNA exhibiting a loss of eyes and head structures, and a morphologically irregular notochord (also see panel F). (D) Dorsal anterior view of an embryo injected with 50 pg sur mRNA exhibiting eyes of unequal sizes. (E) Dorsal anterior view of an embryo injected with 50 pg FoxH1 mRNA exhibiting a single unilateral eye. (F) Enlarged portion of embryo in panel C at the level of the yolk extension. Arrows indicate the abnormal notochord.