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Fig. 2
A single morpholino targets a matched sequence in both tbx2 genes. A–F: Embryos were injected at the one-cell stage with 100 pg of tbx2a-gfp (A–D) or tbx2b-gfp (E,F) RNA which leads to strong fluorescence throughout the embryo at 24 hpf (A,C,E). This signal is completely blocked by co-injection of MO2a (B) or MO2ab (D,F). Note that the previously validated MO2b is not used in this assay, because it is a splice-blocker. However, MO2a does not block expression of the tbx2b-gfp transcript (not shown). For each sample n > 25.
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