ZFIN Image: Putiri et al., 2011, Fig. s10

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Figure Caption

Fig. s10

Expression of a wild-type, but not mutant, form of dhx16 rescues the necrosis associated with the dhx16 zygotic null phenotype. (A–C) Groups of unsorted progeny from two dhx16^hi4049/+ heterozygous parents, uninjected (A), or injected with mRNA encoding the wild-type (B) and maternal-effect mutant (mis^t792) (C) alleles of dhx16. The necrosis phenotype observed in uninjected controls, expected in ¼ of embryos (experimentally observed in 26.0% of embryos, n = 100; asterisks in (A)) is not observed (0%, n = 29) in embryos expressing wild-type Dhx16 (B). Embryos injected with mRNA coding for the product corresponding to the maternal-effect mis^t792 allele (Dlhx16 I->N product) exhibit necrosis embryos at a fraction (25.5%, n = 47) and with a similar phenotypic strength as uninjected siblings (asterisks in (A,C)). Data are compiled from two separate experiments with similar results.

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Reprinted from Developmental Biology, 353(2), Putiri, E., and Pelegri, F., The zebrafish maternal-effect gene mission impossible encodes the DEAH-box helicase Dhx16 and is essential for the expression of downstream endodermal genes, 275-289, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.