Fig. S2
Bmp2b overexpression does not induce ectopic hepatocytes. (A-D) Embryos obtained from crossing wnt2bb-/- with Tg(hs:bmp2b);wnt2bb-/- zebrafish were heat-shocked at 22 (A,B) or 26 (C,D) hpf, and harvested at 73-75 hpf for whole-mount immunostaining. The Tg(fabp10:dsRed,ela3l:EGFP) line was used to reveal hepatocytes (red) and pancreatic acinar cells (green); mAb 2F11 staining reveals the hepatopancreatic duct (blue). Bmp2b overexpression did not induce ectopic hepatocytes but blocked the formation of the pancreatic tail (n=6 out of 6). Brackets mark the extent of the pancreatic tail. Ventral views, anterior upwards. Scale bar: 50 μm.