ZFIN Image: Popgeorgiev et al., 2011, Fig. 3

Image

Figure Caption

Fig. 3 Nrz Knockdown Results in Caspase-Independent Epiboly Arrest

(A) Left panels: activated caspase-3 staining of embryos injected at the one cell stage with egfp mRNA (control), nrzMO (0.5 mM), zbax mRNA, or zbax mRNA plus p35 mRNA. Zbax-expressing embryos were stained at the two to eight cell stage, whereas nrz morphants were labeled at the 30%–50% epiboly stage. Right panels: same embryos stained with Hoechst′s reagent to visualize nuclei. Scale bar: 200 μm.

(B) Mortality rate of control zebrafish embryos injected with p35 and zbax mRNA either alone or in combination and of nrz morphants coinjected or not with p35 mRNA (mean ± SD; three independent experiments).

(C) Bright-field images showing the phenotype of control embryos and nrz morphants injected with p35 mRNA. Scale bar: 500 μm.

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Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Cell, 20(5), Popgeorgiev, N., Bonneau, B., Ferri, K.F., Prudent, J., Thibaut, J., and Gillet, G., The Apoptotic Regulator Nrz Controls Cytoskeletal Dynamics via the Regulation of Ca(2)+ Trafficking in the Zebrafish Blastula, 663-676, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell