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Fig. 5 REST function is required for FBMN migration. (A-C) Maximum projection dorsal views of FBMNs (green) in 42 hpf Tg(islet1:GFP) zebrafish embryos, immunostained for EphA4 to mark r5 (red). FBMN migration is partially disrupted in REST morphants (B) and in mCherry-zhREST4-injected embryos (C), relative to controls (A). Scale bar: 20 μm. (D) The extent of FBMN migration was scored in embryos with disrupted REST function. The number of embryos scored is indicated in parentheses.
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