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Fig. 7 FTI treatment of zprogerin/zlamin A-Δ37 transgenic zebrafish.
(A) External features of zprogerin transgenic zebrafish (30 hpf) without (Control, -FTI) or with FTI treatment (100 μM, +FTI). (B) Amelioration of nuclear morphology by FTI treatment (100 μM, +FTI) in comparison with untreated (-FTI) zprogerin fish. Analysis of the misshapen nuclear morphology was also performed by using different concentrations of FTI in zprogerin transgenic embryos at 30 hpf as shown in right graph. The number of cells with abnormally shaped nuclei were determined by fluorescence microscopy (*P<0.05). (C) Amelioration of muscle phenotype (stained with the F59 antibody) at 24 hpf and reduction of SA-β-gal activity at 3.5 dpf in zprogerin fish embryos by FTI treatment (100 μM, +FTI) in comparison with the untreated condition (-FTI). (D) Western blot analysis of prelamin A accumulations in AB9 zebrafish fibroblasts following FTI treatment, and the quantitation of prelamin A amounts in right graph. The quantifications of intrinsic prelamin A accumulations were shown are the averages from two-independent experiments.