ZFIN Image: Lobbardi et al., 2011, Fig. 3

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Figure Caption

Fig. 3

QkA activity is not required in fast muscle cells. (A) Scheme for the generation of morphant clones. qkA^gt-MO was injected together with GFP RNA at the 8-cell stage and embryos were sorted for the dorsoventral position of the clone at the onset of gastrulation. (B-J) Embryos were immunostained at 24 hpf for fast muscle (F310) and GFP and analyzed by confocal microscopy. (B,C,E,F,H,I) Lateral views, anterior to the left; (D,G,J) corresponding optical sections (merge). (K-M) GFP-labeled WT (K,L) or mutant (M) cells were transplanted into WT (arrows; K,M) or qkA^t31954 homozygotes (arrowheads; L) and analyzed for fast muscle development at 30 hpf. NC, notochord.

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