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Fig. S3
Endothelial cell counts in fixed embryos are comparable with live embryos. Endothelial cells in the BCA/PCS were counted in five live Tg(fli1a:nEGFP)y7 tnnt2a morphants at 48 hpf, then counted again after fixation. Results revealed no difference in cell counts, with an average (mean±s.e.m.) of 41.2±1.35 cells in live embryos versus 40±1.09 cells in fixed embryos. It is difficult to count cells in collapsed vessels, so these images represent the greatest counting challenge. Two-dimensional confocal z-projections, dorsal views, anterior leftwards. Scale bar: 50 μm.
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