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Fig. 9
Figure 10. Destabilized enhanced green fluorescent protein (d2GFP) levels are altered when BMP activity is manipulated. A–C: Overexpression of Bmp2b (B) driven by the inducible hsp70 heat-shock promoter increases d2GFP expression levels relative to endogenous BRE:d2GFP in control larvae (A) at 24 hpf when imaged 6 hours after a 30 minute heat shock at 37°C. Note the loss of the dorsal high-ventral low gradient pattern in the eye which is replaced by a uniformly high level of d2GFP expression. Conversely, inhibition of BMP signaling by hsp70 overexpression of Noggin3 downregulates d2GFP expression (C). The embryo position is indicated by dashed lines. D–I: At 3 dpf, overexpression of Bmp2b increases both d2GFP and pSmad1/5/8 immunoreactivity relative to normal levels, particularly in the jaw (arrow) and pectoral fin (arrowhead). J–O: In the 3 dpf eye, Bmp2b overexpression increased d2GFP expression and pSmad1/5/8 immunoreactivity.