ZFIN Image: Osborn et al., 2011, Fig. S3

Image

Figure Caption

Fig. S3 Cdkn1c knockdown does not affect myod mRNA in presomitic mesoderm

(A). In situ mRNA hybridization for myod alone (left panel) or myod and myog (right panel) in a shha^tbx392/+ incross injected with cdkn1c MOs or vehicle-control. Note the reduced numbers of myog-expressing cells in shha;cdkn1c MO embryo. Dorsal view of flatmounts at 8 som, anterior to top. (B). Western analysis of Myod in 24 hpf embryos injected with cdkn1c MO. A single major band of M_r ~ 44 000 is not reduced relative to β-Tubulin loading control (M_r ~ 55 000). When averaged over three experiments on 16–24 hpf embryos, no significant down-regulation of Myod band was detected after correction for protein loading. Bars: 25 μm.

Acknowledgments

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Reprinted from Developmental Biology, 350(2), Osborn, D.P., Li, K., Hinits, Y., and Hughes, S.M., Cdkn1c drives muscle differentiation through a positive feedback loop with Myod, 464-475, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.