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Fig. S2 (A–E) Blocking reagents for Xenopus, zebrafish, and mouse apobec2. (A) Sequence of the two morpholino oligonucleotides for Xenopus A2 depletion. (B) Sequence of the morpholino oligonucleotide for zebrafish A2 depletion. (C, D) Unilateral PM injection of XA2 MO (10 ng) does not affect antero-posterior development. Dorsal views of control (C) and XA2 MO injection in left PM (D). (E) Effect of different siRNAs (1–3) against mouse A2 mRNA on expression of A2 protein levels in C2C12 cells. siA2.2 was chosen for the experiments in Fig. 5. DM: differentiation medium, GM: growth medium. Lanes 1–5: endogenous A2. Lane 7: overexpressed A2 (CMV-APOBEC2 transfection). Western blot for mouse A2 and tubulin. (F–I) Targeted injections for PM and LPM. Embryos injected at the 4 cell stage with fluorescent control MO (lateral subequatorial in the dorsal left blastomere, G) and Cherry-H2B (lateral subequatorial in the ventral left blastomere, H) were fixed at stage 20. Dorsal views, posterior is down. The same embryo in visible (F), green UV channel (G), red UV channel (H), and overlap of green and red (I).
Reprinted from Developmental Biology, 350(1), Vonica, A., Rosa, A., Arduini, B., and Brivanlou, A.H., APOBEC2, a selective inhibitor of TGFβ signaling, regulates left–right axis specification during early embryogenesis, 13-23, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.