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Fig. S4 NC and endoderm are patterned and specified correctly in alcama morphants. 30 hpf control (A,C,E) and alcama morphants (B,D,F). Tg(fli1:EGFP) larvae stained with Zn5 antibody in red (A,B) show that NC is patterned correctly in absence of alcama. dlx2a (C,D) and nkx2.3 (E,F) expression in NC and endoderm, respectively, reveals correct specification of NC and endoderm. a1 and a2 label pharyngeal arches 1 and 2. Dotted lines highlight pharyngeal pouches. Scale bars: 50 μm.
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Reprinted from Developmental Biology, 349(2), Choudhry, P., Joshi, D., Funke, B., and Trede, N., Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis, 483-493, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.