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Fig. S2 Determination of targeting specificity of eveMO. A) Target sequences of eveMO wild-type (top) and eveMO mutant (bottom) were placed in front of mCherry and cloned into pCS2+ vector. The black line indicates the binding location of eveMO. B) In vitro transcribed eve1WT-mCherry and eve1Mut-mCherry mRNAs were injected either alone or with eveMO into one-cell stage embryos. Bright field and fluorescent images of injected embryos at 6 hpf are shown. Note that eveMO is unable to block the translation of eve1Mut-mCherry mRNAs.
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Reprinted from Developmental Biology, 349(1), Seebald, J.L., and Szeto, D.P., Zebrafish eve1 regulates the lateral and ventral fates of mesodermal progenitor cells at the onset of gastrulation, 78-89, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.