ZFIN Image: Chi et al., 2010, Fig. 1

Image

Figure Caption

Fig. 1

dococ^s226 Mutants exhibit pericardial edema and cardiac failure due to dysynchronous contraction of the ventricle. (A) Brightfield micrographs of 48 hpf WT (WT) and dco^s226 mutant (dco ^-/-) embryos. Black arrow points to pericardial edema. (B and C) Confocal micrographs of 60 hpf Tg(flk1:EGFP)^s843 (B) WT and (C) dco^s226 mutant hearts (endothelium green) stained with rhodamine phalloidin (myocardium red). dco Mutant ventricles fail to contract coordinately and exhibit disorganized chamber walls. White arrowheads point to atrioventricular canal; yellow arrows point to outflow tract; white asterisk denotes aberrant ventricular wall contraction. (D) Genetic map of the dco region. Numbers below SSLP markers indicate recombination events out of 922 diploid embryos examined. (E) Sequencing of cx46 cDNA revealed a C-to-T change at base pair 494 in the s226 mutant allele, resulting in a Thr-to-Ile substitution at residue 165. (F) Schematic diagram illustrating modular structure of Cx46. A connexon consists of four transmembrane domains (M1–M4). The M3 domain is the major pore-lining domain. Red asterisk marks site affected by s226 mutation.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA