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Fig. S1 (A1–A3 and associated close-ups B1–B3) Double fluorescent ISH and Immunostaining between endogenous hcrt mRNA and EGFP protein driven by hcrt promoter as visualized using confocal microscopy on brain sections of adult hcrt:EGFP transgenic fish (reconstructed stacks of 0.5- or 1-μm sections). Note the very good colocalization demonstrating the specificity of the hcrt promoter fragment used in the transgenic lines. (Scale bar, 100 μm A1–A3; 20 μm B1–B3.)
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Full text @ Proc. Natl. Acad. Sci. USA