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Fig. S6 Reduction in Notch-Delta signaling accounts for some srn phenotypes in the retina. A. Retina patterning was examined with immunostaining using antibody Zn5 at 72 hpf. Retina cell patterning appears grossly normal in srn, des, dla and medium dose DAPT treated embryos, but in mib and high does DAPT treated embryos retinal ganglion cell number is reduced, probably due to increased cell death, as previously reported (Bernardos et al., 2005) (8 embryos, 2 carrier pairs were examined). Scale bar = 40 μm. B. Glial cells in the retina were examined after immunostaining with anti-GFAP antibody. In the retina, the number of radially oriented GFAP+ Muller cells is decreased in srn and mib and medium dose DAPT treated embryos, but not in des or dla (8 embryos, 2 carrier pairs were examined). Scale bar = 40 μm. These results suggest that a reduction in Notch-Delta signaling may account for the glial defects observed in srn mutants.