ZFIN Image: Hippe et al., 2009, Fig. S4

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Figure Caption

Fig. S4 Pattern of Gβ1 and Gβ1like mRNA content in zebrafish embryos as analyzed by in situ-hybridization. Expression pattern of Gβ1 (A, dorsal view; C, lateral view) and Gβ1like (B, dorsal view; D, lateral view) mRNA in zebrafish embryos at 72 hpf as detected by whole mount in situ hybridization. Dorsal (E) and lateral view (F) of sense RNA probed embryos as a control. To avoid cross reactions despite high homology, digoxygenin-incorporated antisense RNA probes were directed against sequences in the 5′-untranslated region of the zebrafish gnb1 and gnb1l gene, respectively, in which both genes are largely different. Whole-mount in situ hybridization revealed an identical pattern of Gβ1 or Gβ1like mRNA content.

Acknowledgments

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