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Fig. S5 Whole-mount in situ hybridization and RT-PCR were carried out for wild-type and MO-injected (zFat1MOand zScrib MO) zebrafish embryos. Embryos were hybridized with Survivin anti-sense mRNA at 48 h.p.f using identical conditions and exposure times. Arrowheads indicate regions of the zebrafish embryo with increased levels of Survivin expression in the zScrib MO- and zFat1 MO-injected embryos. The up-regulation of Survivin in zScrib MO- and zFat1 morphants was confirmed by RT-PCR at 48 h.p.f.
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