ZFIN Image: Skouloudaki et al., 2009, Fig. 3

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Fig. 3

Control of YAP1 expression is essential for normal zebrafish pronephros development. (A) Knockdown of zYAP1 by MO (zYAP1 MO) results in pronephric cysts. Lateral view of embryos injected with zYAP1 MO (0.5 pmol, 1.25 pmol, and 2.5 pmol) at 55 h.p.f. A curled tail and shortening of the body (arrows) are observed in the zYAP1 morphants (Upper). At higher concentration, zYAP1 MO injections led to cyst formation (Lower). (B) Cyst formation caused by zFat1 MO was partially rescued by the simultaneous knockdown of zYAP1. (C) Overexpression of human YAP1 (hYAP1) recapitulated the loss-of-function phenotypes (cyst formation) caused by zFat1 knockdown (zFat1 MO). (D) Over-expression of human YAP1 resulted in pronephric cysts in zebrafish embryos. This result was reversed by co-injection of human Scribble RNA (hScrib). (Numbers in parentheses indicate the total number of embryos used in each experiment.)

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Acknowledgments

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