ZFIN Image: Hellman et al., 2010, Fig. 4

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Figure Caption

Fig. 4

foxj1a target genes are up-regulated in response to tubule obstruction. Control expression of foxj1a target genes efhc1 (A), tektin-1 (D), and dnahc9 (G) at 56 hpf is up-regulated by distal pronephric obstruction (B, E, and H). foxj1a knockdown prevented up-regulation in response to tubule obstruction (C, F, and I). Quantitative real-time RT-PCR (J) confirmed up-regulation of foxj1a target gene expression in obstructed tubules (dnahc9 13.3-fold; efch1 3.4-fold, and tektin1 2.6-fold) and inhibition of induction in foxj1a-deficient obstructed tubules. Error bars in J represent range of minimum and maximum fold foxj1a induction relative to control embryos (comparative threshold cycle method) (59).

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Acknowledgments

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